Heterorhabditis and Steinernema EPN offer advantages of mutualistic association with bacteria of the genera Xenorhabdus and Photorhabdus, respectively (Kaya and Gaugler 1993), high virulence, and rapid action to kill their host. Therefore, entomopathogenic nematodes (EPN) have been considered as a potential control agent. Strategus aloeus is a pest with economic impact however its control has been limited to insecticide use and residue handling in renewal palm lots (Cipriano et al. Furthermore, 90 % of this organism’s life cycle is within the larval stages encompassing 267 days (Ahumada et al. The life cycle has an average duration of 307.8 days (egg: 14.5 days instar larvae: First instar larva: 24.4 days, Second instar larva: 41.6 days, Third instar larvae: 200.5 days pupae: 26.8 days). Later on, the saprophylagous larvae feed on the rotting wood, especially in infested renewal palm lots. In this process, the young oil palm bulb is damaged, including the meristematic tissue causing the palm’s death (Ahumada et al. Later, the male opens a lateral perforation, where after 10 days the female arrives to copulate. Oil palm damage is caused generally by the adult male drilling a hole in the ground, with variable length up to 1.5 m, around the young palm’s bulb (younger than 2 years). In Colombia, Meta and Cesar are the two departments with greatest national palm industry participation (Torres-Carrasco et al. Strategus aloeus L (Coleoptera: Melolonthidae), known as “Little bull” or “chiza” is an oil palm insect pest in Colombia, Venezuela, Guyana, Suriname, Northern Brazil, Ecuador, and Peru (Aldana et al. In conclusion, this alternative must be further explored in search of pesticide use and cost reduction, in addition to young palm loss in a plantation. Significant differences were found among treatments (44 ± 5 %, F = 14.676 df = 6. The experiment was repeated three times on different dates. Steinernema sp3 JCL027 was the strain producing the highest mortality rate (19.3 ± 8 %), followed by H. 24, p = 0.0054), registering mortality between 3 and 14 days. Significant differences were observed (F = 11.127, df = 7. indica SL0708 (n = 20) was evaluated under a completely randomized design. colombiense SNI0198, Heterorhabditis bacteriophora HNI0100, H. aloeus third instar larvae exposure to 160 infective juveniles (IJs) per/cm 2 Steinernema sp3 JCL027, S. Entomopathogenic nematodes isolated from the Colombian Andean region were evaluated. Therefore, other alternatives are being sought out. Its management is based on pesticide use or old palm removal in renewal lots. Strategus aloeus L (Coleoptera: Scarabaeidae), known as “Little bull” or oil palm “chiza” is a limiting pest in palm plantation in Cesar Colombia.
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